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5307299 
Journal Article 
A novel method for conversion of valuable BDS intermediates HPBS/Cx-HPBS from DBT/Cx-DBT 
Yu, B; Huang, J; Cai, XF; Liu, XY; Ma, CQ; Li, FL; Xu, P 
2005 
1 
Chinese Chemical Letters
ISSN: 1001-8417
EISSN: 1878-5964 
16 
7 
935-938 
English 
WOS:000231036400025 
A simple recombinant PCR method was used to delete the dszB gene responsible for the slowest step of the Dsz pathway and allow the accumulation of hydroxyphenyl benzene sulfmate (HPBS) in the recombinant E. coli. Using GCMS, HPBS accumulation was confirmed. The recombinant E. coli could also desulfurize Cx-DBT to corresponding Cx-HPBS. The result gave a new insight to BDS process and explored a new method to obtain valuable surfactants from cheap raw materials. 
biodesulfurization; biosurfactant; recombinant PCR