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531938 
Journal Article 
Mass spectrometry of intact ribosomes 
Videler, H; Ilag, LL; McKay, ARC; Hanson, CL; Robinson, CV 
2005 
Yes 
FEBS Letters
ISSN: 0014-5793
EISSN: 1873-3468 
579 
4 
943-947 
English 
The ability to maintain intact ribosomes in the mass spectrometer has enabled research into their changes in conformation and interactions. In the mass spectrometer, it is possible to induce dissociation of proteins from the intact ribosome and, in conjunction with atomic structures, to understand the factors governing their release. We have applied this knowledge to interpret the structural basis for release of proteins from ribosomes for which no high resolution structures are available, such as complexes with elongation factor G and ribosomes from yeast. We also describe how improvements in technology and understanding have widened the scope of our research and lead to dramatic improvements in quality and information available from spectra of intact Abosomes. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. 
mass spectrometry; nanoflow electrospray; ribosome; yeast; Thermus; thermophilus; E. coli; saccharomyces-cerevisiae; angstrom resolution; subunit; dissociation; complex