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5319544 
Journal Article 
Technological and Biological Characterization of Idebenone-Loaded Solid Lipid Nanoparticles Prepared by a Modified Solvent Injection Technique 
Stancampiano, AHS; Acquaviva, R; Carnpisi, A; Vanella, L; Ventura, CA; Puglisi, G; Pignatello, R 
2006 
Yes 
Journal of Biomedical Nanotechnology
ISSN: 1550-7033 
2 
3-4 
253-260 
WOS:000205748300011 
The possibility of preparing solid lipid nanoparticles (SLN) by means of an alternative technique to the most diffused high pressure homogenization and dilution of micro-emulsion was tested. SLN formulations were therefore prepared by a solvent injection procedure (SI), by changing the lipid component (palmitic or stearic acid) and using the lipophilic antioxidant agent idebenone (IDE) as a model drug. SI-SLN showed a good compatibility with the encapsulated drug and interesting sizes (around 160-180 nm) and a negative surface charge (zeta potential around -30 mV) for systemic administration purposes. This method of preparation allowed to obtain smaller nanoparticles and higher drug encapsulation efficiency than a micro-emulsion procedure. SI-SLN were stable upon storage at room temperature for up to 6 months and can be also successfully freeze-dried. A slow and prolonged drug release was observed in vitro from these systems. A biological assay proved that the incorporation of IDE in the SLN matrix maintained or in some cases increased drug protecting activity against free radical-induced oxidative damage of astrocyte cell cultures. 
Solid Lipid Nanoparticles; SLN; Idebenone; Solvent Injection; Antioxidant Activity; APPH; LDH Release; Astrocytes