US EPA

5326122 

Journal Article 

Effects Of Lindane On Liver Mitochondrial Function In The Rat 

Gopalaswamy, UV; Aiyar, AS 

1984 

Yes 

Bulletin of Environmental Contamination and Toxicology
ISSN: 0007-4861
EISSN: 1432-0800 

33 

1 

106-113 

English 

6205709 

The effects of lindane (58899) on respiration and oxidative phosphorylation in rat liver mitochondria were investigated. Liver mitochondria obtained from male Wistar-rats was exposed to 0 to 125 nanomoles (nmol) lindane using 5 millimolar concentrations of succinate or glutamate as substrate. Small aliquots of adenosine-diphosphate (ADP) were added, and respiratory rates were recorded in the presence of ADP and after its depletion. ADP/oxygen ratios and respiratory control indices were calculated. Basal and magnesium stimulated adenosine-triphosphatase (ATPase) activities were determined in the presence of 0 to 200nmol lindane, with or without 50 micromolar solution of 2,4-dinitrophenol (51285). Oxygen uptake on both substrates was unaffected by lindane in the presence of ADP but increased with increasing lindane concentration in the absence of ADP, to 2.5 times control value at 100nmol lindane on succinate and to 6 times control value at 125nmol lindane of glutamate. At the highest lindane concentrations tested, the ADP/oxygen ratio was reduced 45 to 60 percent on both substrates, while the respiratory control index was reduced 56 percent on succinate and 80 percent on glutamate. Basal and magnesium stimulated ATPase activities did not differ under any condition and were decreased by lindane exposure, about 50 percent at 200nmol. 2,4-Dinitrophenol increased ATPase activity 10 times in controls but had no effect on lindane exposed mitochondria. The authors conclude that lindane significantly influences the respiratory chain, the uncoupling of oxidative phosphorylation, and ATPase activity in rat liver mitochondria in-vitro.