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5333899 
Technical Report 
The biosynthesis of cytidine nucleotides and the level of cytochrome P-450 in rat liver after administration of alpha-hexachlorocyclohexane 
Seifert, J; Vacha, J 
1976 
3-4 
359-363 
PESTAB. This paper describes the time course of changes in the utilization of (2-14C)orotic acid in the synthesis of cytidine components of the acid-soluble pool, in liver rRNA and DNA and in levels of cytochrome P-450 after administration of alpha-HCH (alpha-BHC). Male Wistar rats received alpha-HCH orally at 100 mg/kg in olive oil. (2-14C)orotic acid was injected intraperitoneally. This single administration of alpha-HCH did not affect liver weight up to 54 hr after application. The radioactivities of rRNA uridylic acid increased from the 3rd hour after administration of the drug and remained constant for the entire period of observation. Radioactivity of rRNA cytidylic acid decreased after the 5th hour and decreased further throughout the experiment. Similar changes were noted in the pyrimidine components of the acid-soluble pool. Biosynthesis of cytidine nucleotides decreased after 5 hr and continued to decrease throughout the study. Levels of cytochrome P-450 in microsomes did not change in 7 hr after administration, but rose later. Utilization of (2-14C)orotic acid for cytidine nucleotide synthesis of DNA was depressed during the initial intervals after alpha-HCH administration. After 23 hr there was an increase, while later the specific radioactivity again decreased. It is suggested that the changes in the cytidine nucleotide biosynthesis of the liver may be connected with alterations in biosynthesis of microsomal phospholipids after alpha-HCH administration.