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5336890 
Journal Article 
Production of nutritionally desirable fatty acids in seed oil of Indian mustard (Brassica juncea L.) by metabolic engineering 
Bhattacharya, S; Sinha, S; Dey, P; Das, N; Maiti, MK 
2012 
1 
Phytochemistry Reviews
ISSN: 1568-7767
EISSN: 1572-980X 
11 
2-3 
197-209 
WOS:000316407000003 
Development of a designer oilseed crop with improved yield attributes and enhanced nutritional quality for the benefits of mankind and animal husbandry is now achievable with the combination of genetic engineering and plant breeding. In spite of their immense importance, the fatty acid profiles of most oilseed crops are imbalanced that necessitate the use of metabolic engineering strategies to overcome the various shortfalls in order to improve the nutritional quality of these edible oils. Indian mustard (Brassica juncea L.), being one of the important oilseed crops in Indian subcontinent naturally contains similar to 50 % nutritionally undesirable very long chain unsaturated fatty acids (VLCUFAs), e.g. erucic acid (C-22:1). For the purpose of nutritional improvement of B. juncea seed oil, several metabolic engineering strategies have been employed to divert the carbon flux from the production of VLCUFAs to other important fatty acids. Stearic acid, being a saturated but nutritionally neutral fatty acid, is naturally inadequate in most of the conventional oil seeds. Due to its neutral effect on consumer's health and as an important industrial ingredient, increased in planta production of stearic acid in the seed oil not only helps in reduction of production cost but also lessens the trans fatty acid production during commercial hydrogenation process. In this review metabolic engineering strategies to minimize the VLCUFAs along with increased production of stearic acid in the seed oil of B. juncea are discussed, so that further breeding attempts can be made to improve the nutritionally desirable fatty acid profile in the suitable cultivars of this important oilseed crop. 
Edible oil; Fatty acyl-ACP thioesterase; Indian mustard (Brassica juncea); Metabolic engineering; Stearoyl-ACP desaturase