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5361731 
Technical Report 
Effect of 3-trifluoromethyl-4-nitrophenol on in vitro tissue respiration of four species of fish with preliminary notes on its in vitro biotransformation 
Kawatski, JA; McDonald, MJ 
1974 
1 
67-76 
PESTAB. The effect of TFM on fish tissue in vitro respiration, as measured by oxygen consupmtion, was studied. The fish species and tissues examined were: liver of white sucker (Catostomus commersoni), rainbow trout (Salmo gairdneri), largemouth bass (Micropterus salmoides), bluegill sunfish (Lepomis macrochirus) and brain of rainbow trout. TFM (10. 0 ml/l) caused a significant reduction in oxygen consumption by homogenates of the tissues examined. Largemouth bass and white sucker livers were most sensitive, showing significant reduction in oxygen utilization also at 1. 0 mg/l TFM. In the presence of 1. 0 mg/l TFM, the rate of respiration by trout brain increased significantly, but at 0. 5 mg/l there was no significant change in respiration rate. No significant biotransformation of TFM was noted in trout brain homogenates, but all other fish tissues produced at least 2 TFM metabolites when exposed to 1. 0 or 10. 0 mg/l ((SUP)14C)TFM.