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5363406 
Journal Article 
Separation and partial purification of two differentially inducible UDP-glucuronyltransferases from rat liver 
Bock, KW; Von Clausbruch, UC; Josting, D; Ottenwaelder, H 
1977 
Yes 
Biochemical Pharmacology
ISSN: 0006-2952
EISSN: 1873-2968 
Elsevier 
26 
11 
1097-1100 
English 
406903 
WOS:A1977DF95200019 
HEEP COPYRIGHT: BIOL ABS. Conjugation with glucuronic acid by microsomal UDP-glucuronyl-transferase (GT, EC 2.4.1.17) is a major pathway by which the body inactivates and eliminates a wide variety of lipid-soluble xenobiotics and endogenous compounds. Microsomal enzyme inducing agents phenobarbital (PB) and 3-methylcholanthrene (MC) differentially stimulate GT reactions. This was taken as evidence for the existence of multiple GT. To clarify the mechanism of the effect of PB and MC on GT and to characterize the inducible GT, enzyme purification is required. Substantial purification and separation of 1-naphthol-GT and morphine-GT which are preferentially induced by MC and PB, respectively, are reported.