US EPA

5368424 

Journal Article 

EXPERIMENTAL IMMUNOLOGICAL SCREENING-TESTS ON PIDOTIMOD 

Coppi, G; Manzardo, S 

1994 

1 

Arzneimittel-Forschung
ISSN: 0004-4172
EISSN: 1616-7066 

44-2 

12A 

1411-1416 

English 

7857333 

WOS:A1994PZ61500004 

Pidotimod ((R)-3-[(S)-(5-oxo-2-pyrrolidinyl) carbonyl]-thiazolidine-4-carboxylic acid, PGT/1A, CAS 121808-62-6), a new biological response modifier, was administered to immunodepressed (by prednisolone, cyclophosphamide or methotrexate) mice by oral and intraperitoneal route (respectively up to 400 and 200 mg/kg) during several days (up to 9 days). The stimulatory action of the drug on cell-mediated immunity is investigated by measuring the rosette formation by the murine splenic lymphocytes ex vivo, by T- and B-lymphocytes ex vivo proliferative response to mitogens, by dinitrochlorobenzene delayed hypersensitivity induced on the ear, by the graft-versus-host reaction with immunodepressed mice as donors. In all tests pidotimod reveals a potent action in restoring the depressed reactivity. The action of pidotimod on humoral immunity is showed in two tests where the antibody response is induced by a thymus dependent (sheep erythrocytes) or a thymus independent (lipopolysaccharide) antigen. Pidotimod was active in both tests. Macrophage functions, anion superoxide production and the non-stimulated ex vivo chemotaxis reveal that pidotimod significantly reduces the immunodepressant action of prednisolone; particularly in i.p. treated mice the chemotaxis is likely to be restored to the levels of the non-immunodepressed controls. The colloidal china ink blood clearance in vivo in immunodepressed mice, after pidotimod treatment, results similar to that found in the control mice. 

BIOLOGICAL RESPONSE MODIFIERS; CAS 121808-62-6; PGT/1A; PIDOTIMOD, IMMUNOSTIMULATING EFFECTS, PHARMACOLOGY