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5368548 
Journal Article 
Conditioned suppression of contact sensitivity is independent of sympathetic splenic innervation 
Exton, MS; Elfers, A; Jeong, WY; Bull, DF; Westermann, J; Schedlowski, M 
2000 
Yes 
American Journal of Physiology: Regulatory, Integrative and Comparative Physiology
ISSN: 0363-6119
EISSN: 1522-1490 
279 
4 
R1310-R1315 
English 
11003998 
WOS:000089483400021 
The present study investigated the role of sympathetic innervation of the spleen in conditioned suppression of a contact hypersensitivity (CHS) reaction. Behavioral conditioning was achieved by pairing saccharin drinking solution (conditioned stimulus, CS) with injection of cyclosporin A (CsA, 20 mg/kg; unconditioned stimulus, UCS). Four days after sensitization of the animals by application of a 5% 2,4-dinitrochlorobenzene (DNCB) to abdominal skin, the animals were challenged by applying a 1% DNCB solution to the ear. The CHS response was monitored by measuring the degree of ear swelling. Saccharin re-presentation reduced ear swelling to a magnitude that approached that achieved by CsA treatment. Histological examination demonstrated that the conditioned reduction of ear swelling was produced by a reduced leukocyte infiltration of the ear. Prior sympathetic denervation of the spleen did not alter the conditioned suppression of the CHS response. These data indicate that behavioral conditioning using CsA produces alterations of CHS that, unlike conditioned prolongation of heart allograft survival, are independent of sympathetically regulated conditioned alterations in the spleen. 
classical conditioning; cyclosporin 
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