US EPA

536915 

Journal Article 

Effects of nitrobenzenes on DNA damage in germ cells of rats 

Xu, JB; Jing, TS; Yang, L; Sun, ZW; Shi, L 

2006 

Yes 

Chemical Research in Chinese Universities
ISSN: 1005-9040 

22 

1 

29-32 

English 

10.1016/S1005-9040(06)60039-1 

WOS:000235181400008 

The single cell gel electrophoresis (SCGE) technique was used to detect DNA damage in germ cells of rats, which was induced by five tested nitroaromatic compounds. Mixed cultures of Sertoli and germ cells were prepared from the rats testis and their responses to m-dinitrobenzene(m-DNB), 2,4-dinitrotoluene(2,4-DNT), 2,6-dinitrotoluene(2,6-DNT), 4-nitrotoluene(4-NT) and 2,4-dinitroaniline(2,4-DNAn) were studied. The results show that all the five chemicals have a reproductive toxicity. Each dose group and the control group were significantly different (P < 0.01). All of them can lead to the damage to DNA in the germ cells of Kunming male rats in the definite range of concentration (m-DNB: 0.04-25 mu mol/L; 2,4-DNT, 2,6-DNT and 4-NT: 0.032-500 mu mol/L; 2,4-DNAn: 0.8-20 mu mol/L). When the concentration increases, the damage rate will become higher, which shows an evident logarithm dose-effect relationship. 

nitroaromatic compound; Sertoli-germ cell coculture; SCGE; DNA damage; toxicity; qsar