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5372301 
Journal Article 
Induction of glutathione S-transferase activity in Norway spruce by xenobiotics and herbicide safeners 
Schroder, P; Pflugmacher, S 
1996 
Yes 
Angewandte Botanik
ISSN: 0066-1759 
70 
3-4 
97-100 
English 
BIOSIS COPYRIGHT: BIOL ABS. The conjugation of CDNB, DCNB, and CH3I with glutathione is significant in protein extracts of Norway spruce (Picea abies L. Karst.) needles and can be attributed to the activity of glutathione S-transferases (GST, E.C. 2.5.1.18). The GST mediated conjugation of the mentioned substrates can be significantly enhanced by donation of xenobiotics and herbicide safeners in concentrations as low as 50 muM. Inductive effects appear to be coupled to the de-novo synthesis of proteins and may be different for GST isozymes. The conjugation of CDNB was stimulated by donations of CDNB, benoxacor or fluxofenim but the conjugation of DCNB was increased by benoxacor, fenclorim and fluxofenim, whereas CDNB was a potent inhibitor of DCNB-GST. Inductions of DCNB conjugation of 800% above controls were obtained with fluxofenim.