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5373001 
Journal Article 
Electrospinning of casein nanofibers with silver nanoparticles for potential biomedical applications 
Selvaraj, S; Thangam, R; Fathima, NN 
2018 
Yes 
International Journal of Biological Macromolecules
ISSN: 0141-8130
EISSN: 1879-0003 
120 
Pt B 
1674-1681 
English 
30268753 
WOS:000449892800039 
Casein, a major protein content in the milk has been extensively used in drug delivery due to its unique structural features. Fabrication of nanofibers from casein along with nanoparticles for tissue engineering applications has been explored in this study. Nanofibers fabrication is achieved by co-electrospinning of casein with poly (ethylene oxide) in sodium dodecyl sulphate (SDS) aqueous solution. Stabilization of silver nanoparticles has been achieved by the presence of SDS in the nanofiber matrix. The influence of conductivity on the nanofiber fabrication has also been studied. The nanofibrous mats have been characterized using techniques such as scanning electron microscope (SEM) and high resolution-transmission electron microscope (HR-TEM). Antimicrobial properties of the nanofibers have been assessed and the cellular biocompatibility of the material has been evaluated using cultured fibroblast (NIH-3T3) cells. Silver nanoparticles incorporated nanofibers showed good antimicrobial property against both gram negative and gram positive bacteria. In addition, the nanofiber matrix exhibited good biocompatibility for the fibroblast cell proliferation. These results pave the way for extending the use of casein based nanofibers in the skin care applications. 
Nanofibers; Casein; Silver; Fibroblast cells; Biocompatibility; Scaffold