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HERO ID
5384345
Reference Type
Journal Article
Title
Global metabolomic profiling of human synovial fluid for rheumatoid arthritis biomarkers
Author(s)
Carlson, AK; Rawle, RA; Wallace, CW; Adams, E; Greenwood, MC; Bothner, B; June, RK
Year
2019
Is Peer Reviewed?
Yes
Journal
Clinical and Experimental Rheumatology
ISSN:
0392-856X
EISSN:
1593-098X
Volume
37
Issue
3
Page Numbers
393-399
Language
English
PMID
30620276
Web of Science Id
WOS:000473241000007
Abstract
OBJECTIVES:
The objective of this study was to analyse the metabolomic profiles of rheumatoid arthritis synovial fluid to test the use of global metabolomics by liquid chromatography-mass spectrometry for clinical analysis of synovial fluid.
METHODS:
Metabolites were extracted from rheumatoid arthritis (n=3) and healthy (n=5) synovial fluid samples using 50:50 water: acetonitrile. Metabolite extracts were analysed in positive mode by normal phase liquid chromatography-mass spectrometry for global metabolomics. Statistical analyses included hierarchical clustering analysis, principal component analysis, Student's t-test, and volcano plot analysis. Metabolites were matched with known metabolite identities using METLIN and enriched for relevant pathways using IMPaLA.
RESULTS:
1018 metabolites were detected by LC-MS analysis in synovial fluid from rheumatoid arthritis and healthy patients, with 162 metabolites identified as significantly different between diseased and control. Pathways upregulated with disease included ibuprofen metabolism, glucocorticoid and mineralocorticoid metabolism, alpha-linolenic acid metabolism, and steroid hormone biosynthesis. Pathways downregulated with disease included purine and pyrimidine metabolism, biological oxidations, arginine and proline metabolism, the citrulline-nitric oxide cycle, and glutathione metabolism. Receiver operating characteristic analysis identified 30 metabolites as putative rheumatoid arthritis biomarkers including various phospholipids, diol and its derivatives, arsonoacetate, oleananoic acid acetate, docosahexaenoic acid methyl ester, and linolenic acid and eicosatrienoic acid derivatives.
CONCLUSIONS:
This study supports the use of global metabolomic profiling by liquid chromatography-mass spectrometry for synovial fluid analysis to provide insight into the aetiology of disease.
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PFAS 150
Literature Search Update December 2020
PubMed
WOS
Literature Search August 2019
PubMed
Web of Science
N-Ethylperfluorooctanesulfonamide
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