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Citation
Tags
HERO ID
5389731
Reference Type
Journal Article
Title
The role of microRNA-1 targeting of MAPK3 in myocardial ischemia-reperfusion injury in rats undergoing sevoflurane preconditioning via the PI3K/Akt pathway
Author(s)
Hao, YL; Fang, HC; Zhao, HL; Li, XL; Luo, Y; Wu, BQ; Fu, MJ; Liu, W; Liang, JJ; Chen, XH
Year
2018
Is Peer Reviewed?
Yes
Journal
American Journal of Physiology: Cell Physiology
ISSN:
0363-6143
EISSN:
1522-1563
Volume
315
Issue
3
Page Numbers
C380-C388
Language
English
PMID
29741915
DOI
10.1152/ajpcell.00310.2017
Relationship(s)
has retraction
11948557
(Retraction of Vol 315, Pg C 380, 2018)
Abstract
Recent studies have uncovered the vital roles played by microRNAs in regulating cardiac injury. Among them, the cardiac enriched microRNA-1 (miR-1) has been extensively studied and proven to be detrimental to cardiac myocytes. Hence, the current study aimed to explore whether miR-1 affects myocardial ischemia-reperfusion injury (MIRI) in rats undergoing sevoflurane preconditioning and the underlying mechanism. After successful model establishment, rats with MIRI were transfected with mimics or inhibitors of miR-1, or siRNA against MAPK3, and then were injected with sevoflurane. A luciferase reporter gene assay was conducted to evaluate the targeting relationship between miR-1 and MAPK3. Reverse transcription quantitative polymerase chain reaction and Western blot analysis were employed to evaluate the expressions of miR-1, MAPK3, phosphatidylinositol 3-kinase (PI3K), and Akt. Additionally, the concentration of lactate dehydrogenase (LDH) was determined. Cell apoptosis and viability were assessed using TUNEL and cell counting kit-8 assays, and the ischemic area at risk and infarct size were detected using Evans blue and triphenyltetrazolium chloride staining. MAPK3 was found to be the target gene of miR-1. miR-1 expressed at a high level whereas MAPK3 expressed at a low level in MIRI rats. Overexpressing miR-1 or silencing MAPK3 blocked the PI3K/Akt pathway to increase cell apoptosis, ischemic area at risk, and infarct area but decreased cell viability and increased LDH concentration. In contrast, miR-1 downregulation abrogated the effects induced by miR-1 mimics or siRNA against MAPK3. These findings indicate that inhibition of miR-1 promotes MAPK3 to protect against MIRI in rats undergoing sevoflurane preconditioning through activation of the PI3K/Akt pathway.
Tags
PFAS
•
PFAS 150
Literature Search Update December 2020
PubMed
Literature Search August 2019
PubMed
Not prioritized for screening
Sevoflurane
1,1,1,3,3,3-Hexafluoro-2- (fluoromethoxy)propane
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