US EPA

540471 

Journal Article 

Identification of genome organization in the unusual allotetraploid form of Carassius auratus gibelio 

Zhu, HP; Gui, JF 

2007 

1 

Aquaculture
ISSN: 0044-8486
EISSN: 1873-5622 

265 

1-4 

109-117 

English 

10.1016/j.aquaculture.2006.10.026 

WOS:000246128800013 

The unusual allotetraploid form with unequal contribution of chromosome sets was discovered from the gynogenetic offspring of Carassius auratus gibelio stimulated by red common carp sperm. In this study, genomic in situ hybridization (GISH) and fluorescent in situ hybridization (FISH) with 45S rDNA probe are used. The GISH results lead to the identification of species-specific chromosomes, which permits to demonstrate the origin and genome organization in the allotetraploid form. Moreover, chromosome localization of 45S rDNA and co-localizations of 45S rDNA and Cyprinus carpio genomic DNA further confirm that one extra 45S rDNA positive chromosome in the allotetraploid form originates from the paternal haploid genome of C carpio, and other 5 45S rDNA-containing chromosomes are from the maternal genome of Carassius auratus gibelio. And, the correlation between 45 rDNA and the nucleolar organizer regions (NORs) is confirmed by silver nitrate staining. The data provide direct experiment evidence that the allotetraploid actually contains three chromosome sets of Carassius auratus gibelio and one chromosome set of C carpio, and will be a useful genetic material for both basic research and breeding practice. (c) 2006 Elsevier B.V. All rights reserved. 

allotetraploid; Carassius auratus gibelio; genomic in situ; hybridization; fluorescence in situ hybridization; 45S rDNA; genome; organization; nucleolar organizer regions (NORs); in-situ hybridization; silver crucian carp; x common carp; gynogenetic; fish; progeny; hybrids; bloch; chromosomes; clones; localization