Liu, H; Shen, XT; Sun, LN; Chen, XJ
Bioconcentration and biodegradation of dibutyl phthalate(DBP)in Chlorella vulgais were investigated. DBP is a kind of typical en-- docrine-disrupting(EE)substances. Chlorella Vulgaris is a sort of dominant algae in eutrophic waters. Experiments were performed in 250 mL flasks, DBP was analyzed with GC-FID. Experimental results showed that Chlorella vulgaris was able to accumulate DBP significantly, but biodegradation of DBP by the alga was lower. The decrease of DBP in algal solution was described satisfactorily by a first-order kinetic equation. (1)At 25 degree C, with the increase of initial DBP concentrations(0.198, 1.82, 4.85 mg times L super(-1)), DBP accumulation in alga reached the maxima of 1.37 mg times g super(-1)DW at 12 h, 85.9 mg times g super(-1)DW at 1 h, 238 mg times g super(-1)DW at 1 h, respectively. Bioconcentration factors(BCFs)reached the maxima of 8.01x10 super(3) at 12 h, 4.83x10 super(4) at 1 h, 5.36x10 super(4) at 1 h, respectively. And the biodegradation rate constants (k sub(A))were 0.00, 0.80x10 super(-3),0.30x10 super(-3) h super(-1), respectively. For 0.198 mg times L super(-1) DBP, accumulation amount in algal cells was small, which might cause biodegradation to a small extent. For 4.85 mg times L super(-1) DBP, it might be higher toxicity of DBP that inhibited algal growth and activity of enzyme. (2) At similar initial DBP concentration (about 2.00 mg times L super(-1)), accumulation amounts of DBP by algae and BCFs at 13 degree C and 25 degree C both reached the maxima at 1 h. DBP accumulation in alga and BCFs reached the maxima of 24.0 mg times g super(-1)DW and 1.23x10 super(4) at 13 degree C 85.9 mg times g super(-1)DW and 4.83x10 super(4) at 25 degree C respectively. And k sub(A) were 0.60x10 super(-3) and 0.80x10 super(-3) h super(-1), respectively. Studies showed that temperature was one of affecting factors, higher temperature was in favor of biodegradation.
Pollution Abstracts; Aqualine Abstracts; Water Resources Abstracts; Microbiology Abstracts A: Industrial & Applied Microbiology; Microbiology Abstracts C: Algology, Mycology & Protozoology; Biotechnology and Bioengineering Abstracts; ASFA 3: Aquatic Pollution & Environmental Quality; Temperature; Pollution indicators; Toxicity; Toxicity tests; Bioaccumulation; Temperature effects; Mathematical models; phthalates; Water Pollution Effects; Biodegradation; Enzymes; Chlorella; Phthalic acid; Chlorella vulgaris; Eutrophic environments; Eutrophic waters; eutrophic water; Biological Magnification; Algal Growth; Kinetics; Q5 08504:Effects on organisms; A 01320:Microbial Degradation; K 03320:Cell Biology; AQ 00008:Effects of Pollution; SW 3030:Effects of pollution; W 30950:Waste Treatment & Pollution Clean-up; P 6000:TOXICOLOGY AND HEALTH