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5679318 
Journal Article 
Liquid-chromatographic determination of halogenated bisphenols based on intramolecular excimer-forming fluorescence derivatization 
Tsunetomo, S; Yoshida, H; Todoroki, K; Hayama, T; Nohta, H; Kuroki, H; Yamaguchi, M 
2004 
1 
Bunseki Kagaku / Analysis Chemistry
ISSN: 0525-1931 
JAPAN SOC ANALYTICAL CHEMISTRY 
TOKYO 
53 
12 
1501-1505 
Japanese 
WOS:000225755100017 
A selective determination method for halogenated bisphenols containing tetrabromobisphenol A has been developed. This method is based on an intramolecular excimer-forming fluorescence derivatization with a pyrene reagent, 4-(1-pyrene)butanoyl chloride (PBC), followed by reversed-phase chromatography (LC). Bisphenols, containing two phenolic hydroxyl groups in a molecule, were converted to the corresponding dipyrene-labeled derivatives by a reaction with PBC. These derivatives afforded intramolecular excimer fluorescence (440-540 nm), which could be clearly discriminated from the normal fluorescence (360-420 nm) emitted from PBC and monopyrene-labeled derivatives. The PBC derivatives could be separated by reversed-phase LC on an ODS column with isocratic elution using aqueous 99% (v/v) acetonitrile as a mobile phase. The detection limits (signal-to-noise ratio = 3) for halogenated bisphenols were femtomole levels for a 5 μl injection. 
halogenated bisphenol; tetrabromobisphenol A; liquid chromatography; excimcr fluorescence; 4-(1-pyrene)butanoyl chloride; human serum