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5707485 
Journal Article 
Cyanocobalamin Enables Activated Sludge Bacteria to Dechlorinate Hexachloro-1,3-Butadiene to Nonchlorinated Gases 
James, DL; Cord-Ruwisch, R; Schleheck, D; Lee, MJ; Manefield, M 
2008 
Yes 
Bioremediation Journal
ISSN: 1088-9868
EISSN: 1547-6529 
12 
4 
177 
English 
Cyanocobalamin activity in dechlorination of activated sludge bacteria was investigated. Hexachloro-1,3-butadiene (HCBD) was a toxic, aliphatic chlorinated hydrocarbon. It was produced as a by-product from the production of tetrachloroethene, trichloroethene, and carbon tetrachloride. Cyanocobalamin was shown to enhance the rate of carbon tetrachloride degradation both by specific anaerobic bacteria and anaerobic microbial enrichments. Return activated sludge from the local wastewater treatment plant was obtained for use as inoculum. Hydrocarbons and chlorinated hydrocarbons were analysed by headspace subsampling. It was concluded that complete dechlorination of HCBD to C4 gases was possible with anaerobically incubated activated sludge and cyanocobalamin as the electron shuttle. 
Environment Abstracts; BACTERIA; ACTIVATED SLUDGE; WASTEWATER TREATMENT; ADSORPTION; ENZYME ACTIVITY; CHLORINATED HYDROCARBONS; DEGRADATION; ENA 07:General