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HERO ID
576952
Reference Type
Journal Article
Title
Effects of maternal oral morphine consumption on neural tube development in Wistar rats
Author(s)
Nasiraei-Moghadam, S; Sahraei, H; Bahadoran, H; Sadooghi, M; Salimi, SH; Kaka, GR; Imani, H; Mahdavi-Nasab, H; Dashtnavard, H
Year
2005
Is Peer Reviewed?
Yes
Journal
Developmental Brain Research
ISSN:
0165-3806
Volume
159
Issue
1
Page Numbers
12-17
Language
English
PMID
16054236
DOI
10.1016/j.devbrainres.2005.06.012
Web of Science Id
WOS:000232170600002
URL
http://
://WOS:000232170600002
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Abstract
Opiate abuse during pregnancy may result in abnormal nervous system function. In order to evaluate the effects of morphine on the development of the nervous system, the present study focused on the effects of maternal morphine consumption on neural tube development in Wistar rats. Female Wistar rats (250-300 g) were crossed with male rats and coupling time was recorded (embryonic day 0-E0). Experimental groups received 0.1, 0.05, and 0.01 mg/ml of morphine in drinking water daily (14 ml water for each rat). Control group received tap water. On embryonic day 9.5 (E9.5), the animals were anesthetized and the embryos were surgically removed. The embryos were fixed in 10% formalin for 1 week. After this time, weights and lengths (antero-posterior axis--A-P) of the embryos were determined and then tissues were processed, sectioned, and stained in hematoxylin and eosin (H&E). The sections were investigated for neural tube development by light microscope and MOTIC software. The decrease in "A-P" length and embryonic weight for the group that received 0.01 mg/ml morphine was significant. It seems that daily consumption of morphine sulfate could delay neural tube development. In addition, administration of 0.01 mg/ml of morphine led to damage to the regulated neuro-ectoderm layer and its thickness. This study showed that oral morphine consumption leads to neural tube defects, as indicated in the morphometric change and also reduction in weight and length of the embryos. These defects might affect the behavior of the animals.
Keywords
Development; Neural Tube; Addiction; Morphine; Rat
Tags
IRIS
•
Formaldehyde
Nervous system effects
Found
Database search results
Web of Science
Screened
Title/abstract
Specific to other chemicals
Reproductive and Developmental Effects
Screened
Title/abstract
Methodology/therapeutics
Retroactive RIS import
Pre2013
Merged Litsearch Results 100912
Merged LitSearch Results ToxNet 101012
Merged LitSearch Additions 86 Reviews SCREEN
Web of Science Search 100412
2013
HCHON tox Ref Identification 022713
2015
FA DevRepro 072115
Methodology/Therapeutics-Population Criteria
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