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5770583 
Journal Article 
Determination of Polynuclear Aromatic Hydrocarbons Contaminated with Chlorinated Hydrocarbon Pesticides 
Negishi, T 
1978 
Yes 
Bulletin of Environmental Contamination and Toxicology
ISSN: 0007-4861
EISSN: 1432-0800 
Springer-Verlag 
NEW YORK 
19 
5 
545-548 
English 
667383 
WOS:A1978FA78800006 
A sensitive and reliable method was developed for the combined gas chromatography mass spectrometric analysis of benzo(a)pyrene (50328) and other polynuclear aromatic hydrocarbons (PAHs) contaminated with chlorinated hydrocarbon pesticides. Mixtures of standard PAHs and pesticides were dissolved in hexane and partitioned with acetonitrile saturated with hexane. The acetonitrile layer was digested with 1 normal potassium-hydroxide at 100 degrees-C, and the hexane extract of the alkaline mixture was concentrated and analyzed qualitatively for PAHs and pesticides by thin layer chromatography. The eluates of the spots obtained were then analyzed quantitatively by gas chromatography and mass spectrometry. The developed spots of PAHs were almost intact, while the pesticides failed to appear at all. When standard PAHs and pesticides were added to bovine adipose tissue, the recovery values obtained for the PAHs ranged from 80 to 97 percent. Similar analysis of bovine tissue from a commercial source indicated four major and four minor components in the chromatographic system, including compounds tentatively identified on the basis of retention time as PAHs and hydro and methyl derivatives of PAHs. The authors suggest that alkaline digestion removed the interference of the pesticides and conclude that the results obtained indicate that the pollution due to PAHs in animals is metabolized to hydro derivatives and methyl derivatives when the compounds pass through the liver.