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593947 
Journal Article 
Visceral and somatic hypersensitivity in a subset of rats following TNBS-induced colitis 
Zhou, Q; Price, DD; Caudle, RM; Verne, GN 
2008 
1 
Pain
ISSN: 0304-3959
EISSN: 1872-6623 
LIPPINCOTT WILLIAMS & WILKINS 
PHILADELPHIA 
134 
1-2 
9-15 
English 
17481818 
WOS:000252693400004 
Background. Chronic abdominal pain is one of the most common gastrointestinal symptoms experienced by patients. Visceral hypersensitivity has been shown to be a biological marker in many patients with chronic visceral pain. We have previously shown that IBS patients with visceral hypersensitivity also have evidence of thermal hyperalgesia of the hand/foot. Objective. The objective of the current study was to develop an animal model of chronic visceral and somatic hypersensitivity in rats treated with intracolonic trinitrobenzene sulfonic acid. Design. Male Sprague-Dawley rats (200-250 g) were treated with either 20 mg/rat trinitrobenzene sulfonic acid (TNBS, Sigma Chemical Co.) in 50% ethanol (n = 75), an equivalent volume of 50% ethanol (n = 20) or an equivalent volume of saline (n = 20). The agents were delivered with a 24-gauge catheter inserted into the lumen of the colon. Mechanical and thermal behavioral tests were performed using an automated von Frey and Hargreaves device to evaluate somatic hyperalgesia. Colonic distension was performed using an automated distension device to evaluate visceral pain thresholds. All animals were tested 16 weeks after TNBS treatment following complete resolution of the colitis. Results. At 16 weeks, 24% of the treated rats (18/75 rats) still exhibited evidence of visceral as well as somatic hypersensitivity compared to saline- and ethanol-treated rats. Conclusion. Transient colonic inflammation leads to chronic visceral and somatic hypersensitivity in a subset of rats. These findings are similar to the subset of patients who develop chronic gastrointestinal symptoms following enteric infection. 
Irritable bowel syndrome; Animal model; TNBS; Visceral hypersensitivity; Somatic hypersensitivity