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5943219 
Journal Article 
Heat-Induced Antigen Retrieval in Immunohistochemistry: Mechanisms and Applications 
Yamashita, S; Katsumata, O 
2017 
1 
Methods in Molecular Biology
ISSN: 1064-3745
EISSN: 1940-6029 
1560 
147-161 
English 
28155151 
Heat-induced antigen retrieval (HIAR) is an essential technique for current immunohistochemistry. We describe the mechanisms of HIAR and its protocols for formalin-fixed, paraffin-embedded specimens and frozen sections, which are the most popular materials for immunohistochemistry using light microscopy. In addition, we describe the antigen retrieval method for highly masked epitopes and double immunostaining using the horseradish peroxidase (HRP)-labeled antibody method and immunofluorescence method in this chapter.