Health & Environmental Research Online (HERO)


Print Feedback Export to File
5966823 
Journal Article 
Quality Assessment of DNA Extraction Methods from Formalin-Fixed Paraffin-Embedded Tissues 
Ferchichi, M; Jouini, R; Ayari, I; Khanchal, F; Koubaa, W; Chadli-Debbiche, A; Benbrahim, E 
2019 
Yes 
International Journal of Human Genetics
ISSN: 0972-3757 
19 
3 
134-137 
English 
WOS:000489124900002 
DNA extraction from archived formalin-fixed paraffin-embedded (FFPE) tissues represent a great source of information. However, till date, it shows some challenges due to its fragmentation and degradation by formalin. The aim of this study was to assess 3 extraction methods used with 651 FFPE namely QIAamp® DNA FFPE Tissue Qiagen Kit (80%), MagaZorb® DNA Mini-Prep Kit Promega (17.8%) and phenol-chloroform classical extraction (2.2%). Extract concentration mean was 240.05 ng/µl (7-1985 ng/µl) and DNA purity (A260/ A280) mean was 1.98 (1.2 - 4). The classical phenol-chloroform method had the most successful DNA concentration (P < 0.001) but had the worst DNA purity ratio while the Promega and Qiagen kits DNA purity ratio were within the norms (P < 0.001). This assessment showed that even with commercial kits, we should always adjust some parameters to have good DNA extracts which is the first step towards a successful molecular analysis. 
DNA; DNA Extraction; Formalin Fixed Tissues; Pathology; Tumors