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5973667 
Journal Article 
Characterization of Rhodococcus wratislaviensis, a New Gram-Positive Facultative Methylotroph, and Properties of Its C-1 Metabolism 
Kaparullina, EN; Trotsenko, YuA; Doronina, N 
2019 
1 
Microbiology
ISSN: 0026-2617
EISSN: 1608-3237 
88 
1 
46-53 
English 
WOS:000468511500005 
Abstract: A facultative methylotroph, strain 2AzMo (VKM Ac-2782), was isolated from the coastal zone of the Sea of Azov. The cells of the isolate are aerobic gram-positive nonmotile rods. Optimal growth occurs at 1% NaCl, 28°C, and pH 7.5 with 1% СH 3 OH or 0.3% methylamine as the carbon and energy sources. A broad spectrum of polycarbon substrates is also utilized. Sequencing of the 16S rRNA gene of the strain revealed its similarity to Rhodococcus species: 99.9% to R. wratislaviensis IEGM 1112 T (=NCIMB 13082 T ), 99.4% to R. imtechensis IEGM 940 T (=RKJ300 T ), and 99.2% to R. koreensis IEGM 962 T (=DNP505 T ). The level of DNA–DNA homology of strain 2AzMo with R. wratislaviensis IEGM 1112 T (=NCIMB 13082 T ) was 76%, supporting its identification as a strain of this species. However, unlike strain 2AzMo, the type strain R. wratislaviensis IEGM 1112 T , as well as other members of this genus (R. imtechensis IEGM 940 T , R. koreensis IEGM 962 T , and R. opacus IEGM 716 T ), do not grow on methanol and methylamine. Methanol oxidation by R.wratislaviensis 2AzMo is catalyzed by alcohol dehydrogenase, which uses 4-nitroso-N,N-dimethylaniline as an artificial electron acceptor. Methylamine is oxidized by methylamine dehydrogenase and the enzymes of the N-methylglutamate pathway. Formaldehyde is then assimilated via the fructose bisphosphate aldolase variant of the ribulose monophosphate pathway of C 1 metabolism. Ammonium is assimilated by α-ketoglutarate reductive amination and via the glutamate cycle. © 2019, Pleiades Publishing, Ltd. 
Rhodococcus; methylotroph; metabolism; methanol; methylamine