Health & Environmental Research Online (HERO)


Print Feedback Export to File
5975570 
Journal Article 
Enzyme histochemistry on paraffin embedded tissue sections 
Fujimori, T; Mochino, T; Miura, M; Katayama, I 
1981 
1 
Stain Technology
ISSN: 0038-9153 
56 
6 
355-359 
English 
7041331 
To obtain diagnostic enzyme reactions in paraffin embedded tissue sections, we compared four fixatives (buffered formol sucrose, Baker's formol calcium, periodate lysin paraformaldehyde, and buffered formalin acetone) and subsequent acetone dehydration with or without graded concentrations of Triton X-100. Four spleens and 14 lymph nodes were tested for peroxidase, naphthol ASD chloroacetate esterase, acid phosphatase, alpha naphthyl acetate esterase, and alpha naphthyl butyrate. Best results were obtained by a processing method using buffered formalin acetone, Holt's gum sucrose, dehydration in acetone with 0.03% Triton X-100, and paraffin for embedding.