Health & Environmental Research Online (HERO)


Print Feedback Export to File
598764 
Journal Article 
Determination of calcium in milk and water samples by using catalase enzyme electrode 
Akyilmaz, E; Kozgus, O 
2009 
Yes 
Food Chemistry
ISSN: 0308-8146
EISSN: 1873-7072 
115 
1 
347-351 
WOS:000264687300055 
Abstract: A biosensor based on catalase enzyme was developed for the investigation of the effect of calcium ions on the activity of the enzyme. Calcium plays an activator role for the catalase enzyme that catalyses the degradation of hydrogen peroxide to O2 and H2O. Determination method of the effect of calcium ion on the activity of the enzyme was based on the assay of the differences on the responses of the biosensor in the absence and the presence of calcium in the reaction medium. The biosensor had a linear relation to calcium concentrations and good measurement correlation between 1 and 10mM with 1min response time. Tris–HCl buffer (pH 7.0; 50mM) and 37°C were obtained as the optimum working conditions. In the application studies, the biosensor was used determination of calcium level of real samples such as milk, spring and mineral water. [Copyright 2009 Elsevier] Copyright of Food Chemistry is the property of Elsevier Science Publishing Company, Inc. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts) 
Calcium; Hydrogen peroxide; Biosensor; Catalase; Enzyme electrode; Calcium determination