Health & Environmental Research Online (HERO)


Print Feedback Export to File
6011303 
Journal Article 
An evaluation on the Escherichia coli K-12 uvrB/recA DNA repair host-mediated assay: I. In vitro sensitivity of the bacteria to 61 compounds 
Bolcsfoldi, G; Hellmer, L 
1992 
1 
Mutation Research
ISSN: 0027-5107
EISSN: 1873-135X 
272 
2 
145-160 
BIOSIS COPYRIGHT: BIOL ABS. A differential DNA repair test was evaluated in vitro, using derivatives of Escherichia coli K-12 343/113 with the genotype uvrB-/recA- and uvr B+/recA+. The aim of this study was to characterize the sensitivity of the assay to different compounds in vitro and thereby provide information on the usefulness of this end-point as an indicator of genotoxicity in a host-mediated assay. Sixty-one compounds from diverse chemical groups were tested and of these 32 gave a positive result. The results obta compared with results from the Ames test and were in agreement for 49 out of the 61 compounds tested. Chemicals that were detected in this test but negative in the Ames test were 4-aminophenol, catechol, diethylstilbestrol, thioacetamide and thiourea. Seven of the compounds tested gave a negative result in E. coli but were positive in Salmonella. These were 4-aminobiphenyl, benzo(a)pyrene, cyclophosphamide, 1-naphthylamine, N-nitrosobutylpropylamine, quinoline and 2-toluidine. Th 
BIOCHEMISTRY; NUCLEIC ACIDS; PURINES; PYRIMIDINES; BIOPHYSICS; MACROMOLECULAR SYSTEMS; MOLECULAR BIOLOGY; POISONING; ANIMALS, LABORATORY; BACTERIA/GENETICS; VIRUSES/GENETICS; ENTEROBACTERIACEAE; Biochemical Studies-General; Biochemical Studies-Nucleic Acids; Biophysics-Molecular Properties and Macromolecules; Toxicology-General; Genetics of Bacteria and Viruses; In Vitro Studies; Enterobacteriaceae (1992- ); 75888-03-8; 55345-04-5; 25413-64-3; 13073-35-3; 10034-93-2; 7722-84-1; 7647-14-5; 7632-00-0; 7005-18-7; 5307-14-2; 1074-12-0; 759-73-9; 613-13-8; 610-49-1; 512-56-1; 134-32-7; 129-00-0; 123-91-1; 123-30-8; 120-80-9; 120-12-7; 118-92-3; 110-89-4; 108-95-2; 107-22-2; 106-89-8; 103-33-3; 100-75-4; 99-56-9; 95-53-4; 94-59-7; 92-87-5; 92-67-1; 92-52-4; 91-22-5; 90-45-9; 90-04-0; 87-62-7; 86-73-7; 86-30-6; 86-00-0; 77-78-1; 75-07-0; 71-43-2; 67-66-3; 66-27-3; 64-17-5; 63-74-1; 62-56-6; 62-55-5; 60-35-5; 57-13-6; 56-57-5; 56-53-1; 56-23-5; 51-79-6; 50-32-8; 50-18-0; 50-07-7; 50-00-0