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6012624 
Journal Article 
Properties of pertussis toxin mutant PT-9/129G after formaldehyde treatment 
Bugnoli, M; De Magistris, T; Marsili, I; Nencioni, L; Peppoloni, S; Rappuoli, R; Volpini, G 
1991 
Yes 
Infection and Immunity
ISSN: 0019-9567
EISSN: 1098-5522 
59 
2 
625-630 
BIOSIS COPYRIGHT: BIOL ABS. Formaldehyde treatment is a method routinely used to detoxify diphtheria, tetanus, and pertussis toxins as well as other molecules suitable for vaccine production. To investigate whether chemical detoxification alters the immunological properties of vaccine components, we have treated the pertussis toxin mutant PT-9K/129G with formaldehyde and tested the properties of the resulting molecules. Very low concentrations of formaldehyde stabilize the molecule without affecting the physicochemical and immunological parameters. Increasing doses of formaldehyde abolish the mitogenic and hemagglutinating activities of PT-9K/129G. At the same time, the molecule loses the ability to be recognized by a monoclonal antibody specific for a major protective epitope on the S1 subunit of pertussis toxin and its affinity for anti-pertussis toxin polyclonal antibodies is also reduced. In marked contrast, the ability of PT-9K/129G to be recongized by human T-cell clones is not affected by F 
BIOCHEMISTRY; AMINO ACIDS; PEPTIDES; PROTEINS; BIOPHYSICS; MACROMOLECULAR SYSTEMS; MOLECULAR BIOLOGY; AMINO ACIDS/METABOLISM; PEPTIDES/METABOLISM; PROTEINS/METABOLISM; BLOOD CHEMICAL ANALYSIS; BODY FLUIDS/CHEMISTRY; LYMPH/CHEMISTRY; POISONING; ANIMALS, LABORATORY; BACTERIA/PHYSIOLOGY; BACTERIA/METABOLISM; BACTERIA/GENETICS; VIRUSES/GENETICS; MICROBIOLOGICAL TECHNIQUES; IMMUNITY; BACTERIA; GRAM-NEGATIVE AEROBIC BACTERIA; RODENTIA; Biochemical Studies-General; Biochemical Studies-Proteins; Biophysics-Molecular Properties and Macromolecules; Metabolism-Proteins; Toxicology-General; Physiology and Biochemistry of Bacteria; Genetics of Bacteria and Viruses; Microbiological Apparatus; Immunology and Immunochemistry-General; Immunology and Immunochemistry-Bacterial; Medical and Clinical Microbiology-Bacteriology; Gram-negative Aerobic Rods and Cocci-Uncertain Affiliation (1979- ); Caviidae; 70323-44-3