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6022969 
Journal Article 
Synthesis of L-tyrosine by a coupled reaction of serine hydroxymethyl-transferase and beta -tyrosinase 
Hsiao, HY; Lee, TK 
1986 
Yes 
Enzyme and Microbial Technology
ISSN: 0141-0229 
8 
9 
523-526 
Reaction conditions for the synthesis of L-tyrosine from glycine, formaldehyde and phenol in the presence of pyridoxal 5'-phosphate and tetrahydrofolate by a coupled reaction of serine hydroxymethyltransferase and beta -tyrosinase were examined. A recombinant strain of Klebsiella aerogenes and Erwinia herbicola (ATCC 21434) were used as the source of serine hydroxymethyltransferase and beta -tyrosinase, respectively. Phenol and formaldehyde were fed into the reaction vessel depending on the demand of the reaction to minimize the inactivation of the enzymes by these chemicals. The reaction mixture (500 ml) containing 0.25 M glycine, 0.5 mM pyridoxal 5'-phosphate, 0.056 M beta -mercaptoethanol, 1.7 mM tetrahydrofolate, an initial phenol concentration of 0.32% and the cells was maintained at pH 7.0 and 37 degree C. After 16 h, 26.3 g L-tyrosine per litre were produced at 77.3% yield and 61.4% conversion based on glycine. 
synthesis; coupling; serine hydroxymethyltransferase; tyrosine phenol-lyase; tyrosine; Erwinia herbicola; Klebsiella aerogenes; Biotechnology and Bioengineering Abstracts; Microbiology Abstracts A: Industrial & Applied Microbiology; Microbiology Abstracts B: Bacteriology; W 30405:Amino acids; A 01002:Acids, amino acids, peptides & proteins; J 02727:Amino acids, peptides and proteins