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6025975 
Journal Article 
Biological and biochemical activities of a toxoid or erythrogenic toxin type A 
Gerlach, D; Holm, SE; Knoell, H; Koehler, W; Wagner, B 
1988 
269 
4 
468-478 
A toxoid of erythrogenic toxin type A (ET A) was prepared by formaldehyde treatment. Already 15 min after exposure to formaldehyde in isoelectric focusing the ET A band at pH 5.2 shifted to a band at pH 4.5. In Ouchterlony double diffusion test ET A and its toxoid were found to be identical, in fused rocket immuno-electrophoresis a reaction of partial identity was seen. Formaldehyde treatment of ET A resulted in an apparent increase of electrophoretic mobility. In contrast to ET A, its toxoid is non-mitogenic, non-pyrogenic and has lost its ability to induce delayed type hypersensitivity. Binding of ET A toxoid to human peripheral lymphocytes is of the same magnitude as binding of gold-labelled ET A. 
toxoids; Streptococcus pyogenes; characterization; erythrogenic toxin; Toxicology Abstracts; Microbiology Abstracts B: Bacteriology; J 02823:In vitro and in vivo effects; X 24171:Microbial