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HERO ID
6028616
Reference Type
Journal Article
Title
CRUDE EXTRACT OF DESMODIUM GANGETICUM INDUCES HUMAN A549 LUNG CANCER CELL DEATH THROUGH CELL-CYCLE ARREST IN G sub(1) PHASE
Author(s)
Chen, YF; Lu, YH; Tsai, HY
Year
2008
Is Peer Reviewed?
Yes
Journal
Anticancer Research
ISSN:
0250-7005
EISSN:
1791-7530
Volume
28
Issue
5C
URL
https://search.proquest.com/docview/20181740?accountid=171501
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Abstract
Background: Desmodium gangeticum, Leguminosae, has been widely used as a traditional herb in Taiwan and other countries. In past decades, it has been reported to have anti-inflammatory activity in carrageenan-induced inflamed rats, and to improve the severity of myocardial infarction and anti-ulcer potential in pyloric ligation and histamine induced gastric ulcer in rats and pigs. In this study, we explored its anti-nociceptive and antitumor activities in lung cancer cells. Methods: As an in vivo test, the formalin-induced nociceptive behavior was employed. In vitro, the growth of various various bacteria was evaluated. The MTT viability assay was used for the evaluation of growth inhibition in In lung cancer cells. Flow cytometric analysis and Western blot were used to detect the cell cycle arrest and apoptosis in Desmodium gangeticum treated lung cancer cells. Results: In this preliminary study, Desmodium gangeticum inhibited the biting and licking behavior induced by formalin in a dose-dependent manner. Additionally, it was shown that Desmodium gangeticum could completely inhibit the growth of Pseudomonas and partly that of E. coli at higher concentrations. However, it had no effective on the Klebsiella species. Additionally, Desmodium gangeticum dose-dependently inhibited cell viability and induced G sub(1) phase arrest with down-regulation of cyclin A and B1, and up-regulation of P21, P27 in lung cancer cells. Conclusion: Desmodium gangeticum exhibited anti-nociceptive and anti-bacterial effects. It also inhibited cell viability and induced cell cycle arrest in G sub(1) phase in lung cancer cells.
Keywords
Microbiology Abstracts B: Bacteriology; Genetics Abstracts; Myocardial infarction; Klebsiella; Flow cytometry; Pain perception; Antiinflammatory agents; Lung cancer; Escherichia coli; Licking behavior; Inflammation; Pseudomonas; Desmodium; Western blotting; Formaldehyde; Histamine; Cyclin A; Cell cycle; Antitumor activity; Apoptosis; G1 phase; G 07770:Bacteria; J 02340:Antibiotics & Antimicrobials
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