US EPA

603616 

Journal Article 

Hydrogen Peroxide Biosensor with a Supramolecular Layer-by-Layer Design 

Conrado, C; Juan CMa; Roberto, C; Madyu, M; Belkis, C; Javier Hn; Maria, AL; Maria ASn; Reynaldo, V 

2008 

1 

Langmuir
ISSN: 0743-7463
EISSN: 1520-5827 

24 

15 

7654-7657 

A new sensor design is reported for the construction of an amperometric enzyme biosensor toward H 2O 2. It was based in the supramolecular immobilization of alternating layers of horseradish peroxidase (either modified with 1-adamantane or β-cyclodextrin-branched carboxymethylcellulose residues) on Au electrodes coated with polythiolated β-cyclodextrin polymer. The analytical response of the electrodes, using 1 mM hydroquinone as an electrochemical mediator, increases when the number of enzyme layers increases. The biosensor having three enzyme layers showed a sensitivity of 720 μA/M cm 2and a detection limit of 2 μM and retained 96% of its initial activity after 30 days of storage. The host−guest supramolecular nature of the immobilization method was confirmed by cyclic voltammetry. [ABSTRACT FROM AUTHOR] Copyright of Langmuir is the property of American Chemical Society and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts) 

HYDROGEN peroxide; BIOSENSORS; CYCLODEXTRINS; ELECTRODES; POLYMERS; VOLTAMMETRY; ADAMANTANE