US EPA

604991 

Journal Article 

Free radical activity and lipid soluble anti-oxidant vitamin status in patients with long-term ileal pouch-anal anastomosis 

El Muhtaseb, MS; Talwar, D; Duncan, A; St J O’reilly, D; McKee, RF; Anderson, JH; Foulis, A; Finlay, IG 

2009 

Yes 

Colorectal Disease
ISSN: 1462-8910
EISSN: 1463-1318 

11 

1 

67-72 

Objective Ileal pouch-anal anastomosis (IPAA) is the operation of choice for patients with ulcerative colitis. Free radical activity and the status of lipid soluble antioxidant vitamins have not been previously assessed in patients with IPAA. The aim of the present study was to measure the plasma concentrations of lipophyllic antioxidants and free radical activity in IPAA patients and compare them with normal subjects. Method Forty-eight IPAA patients and 50 healthy controls were studied. A dietary assessment of vitamin E (α-tocopherol) and carotene was undertaken and plasma antioxidant status was assessed. Plasma malondialdehyde (MDA) was measured to assess the extent of free radical damage. In IPAA patients, association between the degree of inflammation in the pouch mucosa and the plasma concentration of lipophyllic antioxidants and extent of free radical activity was investigated. Results The dietary intake of carotene was similar in both groups. Intake of vitamin E was significantly lower in patients than controls ( P = 0.01). In the IPAA group plasma concentrations of α-carotene, β-carotene and lycopene were significantly lower ( P < 0.001) and α-tocopherol:cholesterol ratio significantly higher ( P < 0.001). Free radical damage was significantly greater in patients than controls ( P < 0.01). There were no significant correlations between the degree of inflammation in the pouch and plasma concentrations of MDA, carotenoids, α-tocopherol:cholesterol ratio or intake of vitamins. Conclusion Compared with normal subjects, patients with IPAA have significantly lower plasma concentrations of lipophyllic antioxidants α-carotene, β-carotene and lycopene and higher free radical activity suggesting increased oxidative stress. These differences do not appear to be related to diet and do not correlate with histological severity of pouch inflammation. [ABSTRACT FROM AUTHOR] Copyright of Colorectal Disease is the property of Blackwell Publishing Limited and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts) 

FREE radical reactions; ANTIOXIDANTS; COLON (Anatomy) -- Diseases; ULCERATIVE colitis; INFLAMMATORY bowel diseases; PHYSIOLOGICAL aspects; TREATMENT; carotenoids; free radicals; ileal pouches