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607852 
Journal Article 
Giardia duodenalis: Effects of an ozonized sunflower oil product (Oleozon®) on in vitro trophozoites 
Hernández, F; Hernández, D; Zamora, Z; Díaz, M; Ancheta, O; Rodriguez, S; Torres, D 
2009 
Yes 
Experimental Parasitology
ISSN: 0014-4894 
121 
3 
208-212 
Abstract: The ozonized sunflower oil product (Oleozon®) was investigated to explore its cytotoxic activity on Giardia duodenalis in vitro cultivated trophozites. Oleozon® produced inactivation of Giardia trophozoites in a dose- and cell density-dependent manner. Thirty microliter of Oleozon® with peroxide index value of 500 equivalent-mmol of activated oxygen per kilogram were used to achieve a 100% inhibition (<−4.00 log unit) of trophozoites from an initial inoculum of 15×104 cells. This potent effect was confirmed by transmission electron microscopy where morphological deterioration of superficial structures mainly in the ventral disc, and formation of a great number of micro vesicles in the cytoplasm were found. We concluded that a direct chemical-oxidation attack by the active substances from Oleozon® is one of the causes of the parasitocidal effect of this product. We suggest that the dose and cell density-dependent effect must be taken into account when prescription of this product for giardiasis treatment in humans. [Copyright 2009 Elsevier] Copyright of Experimental Parasitology is the property of Academic Press Inc. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts) 
TRANSMISSION electron microscopy; GIARDIA; SUNFLOWER seed oil; CELL-mediated cytotoxicity