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6129570 
Journal Article 
Acetoclastic methanogenesis led by Methanosarcina in anaerobic co-digestion of fats, oil and grease for enhanced production of methane 
Kurade, MB; Saha, S; Salama, ES; Patil, SM; Govindwar, SP; Jeon, BH 
2019 
Yes 
Bioresource Technology
ISSN: 0960-8524
EISSN: 1873-2976 
272 
351-359 
English 
30384210 
WOS:000451625700045 
Fats, oil and grease (FOG) are energy-dense wastes that substantially increase biomethane recovery. Shifts in the microbial community during anaerobic co-digestion of FOG was assessed to understand relationships between substrate digestion and microbial adaptations. Excessive addition of FOG inhibited the methanogenic activity during initial phase; however, it enhanced the ultimate methane production by 217% compared to the control. The dominance of Proteobacteria was decreased with a simultaneous increase in Firmicutes, Bacteriodetes, Synergistetes and Euryarchaeota during the co-digestion. A significant increase in Syntrophomonas (0.18-11%), Sporanaerobacter (0.14-6%) and Propionispira (0.02-19%) was observed during co-digestion, which substantiated their importance in acetogenesis. Among methanogenic Archaea, the dominance of Methanosaeta (94%) at the beginning of co-digestion was gradually replaced by Methanosarcina (0.52-95%). The absence/relatively low abundance of syntrophic acetate oxidizers and hydrogenotrophic methanogens, and dominance of acetoclastic methanogens suggested that methane generation during co-digestion of FOG was predominantly conducted through acetoclastic pathway led by Methanosarcina.