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Citation
Tags
HERO ID
6130942
Reference Type
Journal Article
Subtype
Review
Title
Complete solubilization of formalin-fixed, paraffin-embedded tissue may improve proteomic studies
Author(s)
Shi, SR; Taylor, CR; Fowler, CB; Mason, JT
Year
2013
Is Peer Reviewed?
1
Journal
Proteomics. Clinical Applications
ISSN:
1862-8346
EISSN:
1862-8354
Volume
7
Issue
3-4
Page Numbers
264-272
Language
English
PMID
23339100
DOI
10.1002/prca.201200031
Web of Science Id
WOS:000320554100007
Abstract
Tissue-based proteomic approaches (tissue proteomics) are essential for discovering and evaluating biomarkers for personalized medicine. In any proteomics study, the most critical issue is sample extraction and preparation. This problem is especially difficult when recovering proteins from formalin-fixed, paraffin-embedded (FFPE) tissue sections. However, improving and standardizing protein extraction from FFPE tissue is a critical need because of the millions of archival FFPE tissues available in tissue banks worldwide. Recent progress in the application of heat-induced antigen retrieval principles for protein extraction from FFPE tissue has resulted in a number of published FFPE tissue proteomics studies. However, there is currently no consensus on the optimal protocol for protein extraction from FFPE tissue or accepted standards for quantitative evaluation of the extracts. Standardization is critical to ensure the accurate evaluation of FFPE protein extracts by proteomic methods such as reverse phase protein arrays, which is now in clinical use. In our view, complete solubilization of FFPE tissue samples is the best way to achieve the goal of standardizing the recovery of proteins from FFPE tissues. However, further studies are recommended to develop standardized protein extraction methods to ensure quantitative and qualitative reproducibility in the recovery of proteins from FFPE tissues.
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