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6134871 
Journal Article 
Bisulfite sequencing of DNA 
Darst, RP; Pardo, CE; Ai, L; Brown, KD; Kladde, MP 
2010 
Chapter 7 
Unit 7.9.1-Unit 7.917 
English 
20583099 
Exact positions of 5-methylcytosine (m(5)C) on a single strand of DNA can be determined by bisulfite genomic sequencing (BGS). Treatment with bisulfite ion preferentially deaminates unmethylated cytosines, which are then converted to uracil upon desulfonation. Amplifying regions of interest from deaminated DNA and sequencing products cloned from amplicons permits determination of methylation at single-nucleotide resolution along single DNA molecules, which is not possible with other methylation analysis techniques. This unit describes a BGS technique suitable for most DNA sources, including formaldehyde-fixed tissue. Considerations for experimental design and common sources of error are discussed.