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Citation
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HERO ID
6134871
Reference Type
Journal Article
Title
Bisulfite sequencing of DNA
Author(s)
Darst, RP; Pardo, CE; Ai, L; Brown, KD; Kladde, MP
Year
2010
Volume
Chapter 7
Page Numbers
Unit 7.9.1-Unit 7.917
Language
English
PMID
20583099
DOI
10.1002/0471142727.mb0709s91
Abstract
Exact positions of 5-methylcytosine (m(5)C) on a single strand of DNA can be determined by bisulfite genomic sequencing (BGS). Treatment with bisulfite ion preferentially deaminates unmethylated cytosines, which are then converted to uracil upon desulfonation. Amplifying regions of interest from deaminated DNA and sequencing products cloned from amplicons permits determination of methylation at single-nucleotide resolution along single DNA molecules, which is not possible with other methylation analysis techniques. This unit describes a BGS technique suitable for most DNA sources, including formaldehyde-fixed tissue. Considerations for experimental design and common sources of error are discussed.
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