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6152960 
Journal Article 
Significance of metal ions, solvents and surfactants to improve the xylan degrading behavior of β-1,4-D-xylanohydrolase from Geobacillus stearothermophilus KIBGE-IB29 
Aman, A; Bibi, Z; Irum Us, S; Muhammad, W; Nawaz, MA; Shah Ali Ul, Q 
2019 
Biocatalysis and Agricultural Biotechnology
ISSN: 1878-8181 
17 
242-246 
Biochemical characterization of the partially purified β-1,4-D-xylanohydrolase from Geobacillus stearothermophilus KIBGE-IB29 revealed its higher catalytic activity and stability in the presence of industrially used metal ions, surfactants and organic solvents. The β-1,4-D-xylanohydrolase displayed 1.85, 1.28 and 1.14 fold increase in its catalytic activity when incubated with K+, Na+ and Cs+ ions, respectively for 60.0 min. The enzymatic activity enhanced up to 171% and 242% when 1.0 mM concentration of Ca2+ and Mg2+ ions was used respectively. An increase in concentration up to 5.0 mM stimulated the β-1,4-D-xylanohydrolase activity up to 3.6 fold in case of ethanol and 1.95 fold with methanol. Formaldehyde, chloroform, Tween 80 and Triton X-100 drastically enhanced the β-1,4-D-xylanohydrolase activity. Furthermore, 2.9 fold increase in activity was noticed with 1.0 mM SDS whereas, EDTA completely inhibited the enzymatic activity. Such an excellent characteristics of β-1,4-D-xylanohydrolase indicate its broad potential to be used in paper and textile industries. 
Characterization; Industrial use; Metal ions; Solvents; Stability; Xylanohydrolase; article; calcium; catalytic activity; chloroform; EDTA (chelating agent); endo-1,4-beta-xylanase; enzyme activity; ethanol; formaldehyde; Geobacillus stearothermophilus; magnesium; methanol; octoxynol; polysorbates; potassium; surfactants; textile industry