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6155475 
Journal Article 
Entrapment of alpha -Chymotrypsin into Hollow Polyelectrolyte 
Antipov, AA; Larionova, NI; Lvov, Y; Mohwald, H; Sukhorukov, GB; Tiourina, OP 
2001 
1 
Macromolecular Bioscience
ISSN: 1616-5187 
1 
5 
209-214 
Stable hollow polyelectrolyte capsules were produced by the layer-by-layer assembling of non-biodegradable polyelectrolytes - poly(allylamine) and poly(styrenesulfonate) on melamine formaldehyde microcores followed by the core decomposition at low pH. A proteolytic enzyme, -chymotrypsin, was encapsulated into these microcapsules with high yields of up to 100%. The encapsulation procedure was based on the protein adsorption onto the capsule shells and on the pH-dependent opening and closing of capsule wall pores. The protein in the capsules retained a high activity, and thermo- and storage stability. The nanostructured polyelectrolyte shell protected the proteinase from a high molecular weight inhibitor. Such enzyme-loaded capsules can be used as microreactors for biocatalysis. 
Biotechnology and Bioengineering Abstracts; Encapsulation; Macromolecules; Proteinase; Proteolytic enzymes; Polyelectrolytes; Shelf life; Decomposition; pH effects; Formaldehyde; Adsorption; alpha -Chymotrypsin; microcapsules; W 30900:Methods