Jump to main content
US EPA
United States Environmental Protection Agency
Search
Search
Main menu
Environmental Topics
Laws & Regulations
About EPA
Health & Environmental Research Online (HERO)
Contact Us
Print
Feedback
Export to File
Search:
This record has one attached file:
Add More Files
Attach File(s):
Display Name for File*:
Save
Citation
Tags
HERO ID
6178112
Reference Type
Journal Article
Title
Preparation of monolayer smears from paraffin-embedded tissue for image cytometry
Author(s)
van Driel-Kulker, AM; Mesker, WE; van Velzen, I; Tanke, HJ; Feichtinger, J; Ploem, JS
Year
1985
Is Peer Reviewed?
Yes
Journal
Cytometry
ISSN:
0196-4763
EISSN:
1097-0320
Volume
6
Issue
3
Page Numbers
268-272
Language
English
PMID
3888557
DOI
10.1002/cyto.990060314
Abstract
A method is described for the preparation of monolayer smears from paraffin-embedded tissue suitable for automated image analysis and DNA measurements. The proposed technique uses enzyme treatment and syringing for cell dispersal. Slide preparation is performed by centrifugal cytology. After Feulgen staining the quality of the monolayer smears is sufficiently high to enable visual morphologic evaluation. Automated DNA measurements using the Leyden television analysis system (LEYTAS) show coefficients of variation (CV) of 4.5% for the diploid cell population of the suspended tissue. This is approximately the same as the CV in fresh material from the same tumor. Formalin fixed trout red blood cells are used as reference cells. By applying image cytometry to paraffin-embedded tissue this method allows retrospective studies of, for instance, the significance of DNA content with regard to the behavior of a tumor.
Home
Learn about HERO
Using HERO
Search HERO
Projects in HERO
Risk Assessment
Transparency & Integrity