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6178112 
Journal Article 
Preparation of monolayer smears from paraffin-embedded tissue for image cytometry 
van Driel-Kulker, AM; Mesker, WE; van Velzen, I; Tanke, HJ; Feichtinger, J; Ploem, JS 
1985 
Yes 
Cytometry
ISSN: 0196-4763
EISSN: 1097-0320 
268-272 
English 
A method is described for the preparation of monolayer smears from paraffin-embedded tissue suitable for automated image analysis and DNA measurements. The proposed technique uses enzyme treatment and syringing for cell dispersal. Slide preparation is performed by centrifugal cytology. After Feulgen staining the quality of the monolayer smears is sufficiently high to enable visual morphologic evaluation. Automated DNA measurements using the Leyden television analysis system (LEYTAS) show coefficients of variation (CV) of 4.5% for the diploid cell population of the suspended tissue. This is approximately the same as the CV in fresh material from the same tumor. Formalin fixed trout red blood cells are used as reference cells. By applying image cytometry to paraffin-embedded tissue this method allows retrospective studies of, for instance, the significance of DNA content with regard to the behavior of a tumor.