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6202150 
Journal Article 
The influence of L-ascorbic acid on the antibacterial-toxic activity of aflatoxins on adsorbent layer 
Móricz, AM; Ott, PG; Billes, F; Otta, KH; Tyihák, E 
2007 
Yes 
Journal of Applied Microbiology
ISSN: 1364-5072
EISSN: 1365-2672 
103 
6 
2525-2532 
English 
18045436 
WOS:000251251200055 
AIMS: To substantiate the role of formaldehyde (HCHO) and its reaction products in the mechanism of the antibacterial-toxic effect of aflatoxins B1 (AFB1), B2, G1 and G2.

MATERIALS AND RESULTS: Toxins were separated by overpressured layer chromatography, which was followed by biological evaluation directly on the adsorbent layer (BioArena system with Pseudomonas savastanoi pv. phaseolicola indicator bacteria). HCHO formed in this system was eliminated with exogenously added capturer molecule dimedone and L-ascorbic acid (AA) and measured as the adduct of dimedone and HCHO. The amount of HCHO was higher in the toxin-containing spots, particularly in the most toxic AFB1 spot, compared to a toxinless background. 0.1 mg ml(-1)AA augmented, 0.2 mg ml(-1) dimedone or 0.5 and 1 mg ml(-1) AA reduced the antibacterial effect of all four aflatoxins.

CONCLUSION: The antibacterial-toxic effect of aflatoxins may be mediated by HCHO (and/or its reaction products) generated from bound HCHO forms in the bacterial cells. Basis of antibacterial-toxic activity of the four aflatoxins appears the same.

SIGNIFICANCE AND IMPACT OF THE STUDY: Involvement of HCHO as a key molecule in the effect of aflatoxins indicates a totally new mechanism of action of these dangerous molecules. The BioArena system is useful to dissect the mode of action of antimicrobial compounds from different biological matrices. 
aflatoxins; BioArena; bioautography; formaldehyde; L-ascorbic acid; OPLC