Health & Environmental Research Online (HERO)


Print Feedback Export to File
6209562 
Book/Book Chapter 
[18] Liposome-encapsulated hemoglobin as an artificial oxygen-carrying system 
Farmer, MC; Gaber, BP 
1987 
1 
Methods in Enzymology
ISSN: 0076-6879
EISSN: 1557-7988 
Academic Press 
Methods in Enzymology 
149 
Elsevier 
184-200 
Publisher Summary This chapter discusses a method for encapsulating a high concentration of hemoglobin in liposomes for use as a high-capacity artificial oxygen-carrying system. This chapter also explains many of the biophysical properties of the liposomes as a function of procedural variables in the iterative process of developing an improved technique. It has been verified in detail that the functional properties of the encapsulated hemoglobin are unchanged. Extensive characterization of the physiological response of mice and rats to transfusion of liposome-encapsulated hemoglobin is underway. A scaled-up preparative method for large-scale testing of the efficacy of this artificial oxygen-carrying system is discussed. The methane extrusion method outlined has proven to be quite successful on a laboratory scale, and should have wide applicability for encapsulating hemoglobin and other proteins in phospholipid membranes. This chapter encourages other investigators to explore novel applications of this method. 
Methods in Enzymology 
9780121820497