US EPA

6217931 

Journal Article 

Interactions between homopolyribonucleotides and bacterial ribonucleic acid in solutions of high ionic strength: Part I 

Grunberg-Manago, M; Guérin, MF; Hayes, DH 

1966 

Yes 

Journal of Molecular Biology
ISSN: 0022-2836
EISSN: 1089-8638 

18 

3 

477-498 

10.1016/S0022-2836(66)80038-4 

http://www.sciencedirect.com/science/article/pii/S0022283666800384
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Synthetic homopolyribonucleotides (poly U, poly C and poly G, but not poly A) form complexes with Escherichia coli ribosomal RNA in the presence of sufficient concentrations of monovalent (0·6 m), or divalent (0·01 M) cations. The formation and properties of these complexes have been studied using the technique of sedimentation on sucrose gradients. Both subunits of ribosomal RNA form complexes with poly IT, poly C and poly G. Complex formation takes place more readily with 23 s than with 16 s ribosomal RNA, and yields products with much larger sedimentation coefficients. Neither brief heating of polyribonucleotides or ribosomal RNA, nor extensive thermal degradation of the latter, prevents complex formation. Formaldehyde-treated ribosomal RNA can form complexes with poly C but not with poly U. An interpretation of these observations, based on interactions between complementary bases in the complex-forming polynucleotides is proposed.