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623370 
Journal Article 
Enhanced zinc and cadmium tolerance and accumulation in transgenic Arabidopsis plants constitutively overexpressing a barley gene (HvAPX1) that encodes a peroxisomal ascorbate peroxidase 
Xu, W; Shi, W; Liu, F; Ueda, A; Takabe, T 
2008 
Yes 
Botany
ISSN: 1916-2790
EISSN: 1916-2804 
86 
6 
567-575 
WOS:000256118800004 
Ascorbate peroxidase (APX) plays an important role in oxidative stress metabolism in higher plants. To determine the role of APX in protection against excessive-zinc-induced oxidative stress, transgenic Arabidopsis plants constitutively overexpressing a peroxisomal ascorbate peroxidase gene (HvAPX1) from barley were analyzed. In this study, we found that transgenic plants were more tolerant to zinc stress than wild-type plants. Under zinc stress, the concentration of hydrogen peroxide and malondialdehyde accumulation were higher in wild-type plants than in transgenic plants. Therefore, the mechanism of zinc tolerance in transgenic plants may be due to reduced oxidative stress damage. Under zinc stress, the activities of APX were significantly higher in transgenic plants than in wild-type plants. We also found that the zinc accumulation in the shoots were much higher in transgenic plants than in wild-type plants under zinc stress. In addition, we found that compared with wild-type plants, transgenic plants were more tolerant to excessive cadmium stress and accumulated more cadmium in shoots. These results suggest that HvAPX1 plays an important role in zinc and cadmium tolerance, and might be a candidate gene for developing high-biomass tolerant plants for phytoremediation of zinc- and cadmium-polluted environments. L’APX (peroxydase ascorbique) joue un rôle important dans le métabolisme oxydatif du stress chez les plantes supérieures. Afin de déterminer le rôle de l’APX dans la protection contre le stress oxydatif causé par un excès de zinc, les auteurs ont analysé des plants d’Arabidopsis surexprimant de façon constitutive un gène de la peroxydase ascorbique peroxysomal (HvAAPX1) provenant de l’orge. Dans leurs études, les auteurs constatent une meilleure tolérance au stress causé par le zinc chez les plantes transgéniques... [ABSTRACT FROM AUTHOR] Copyright of Botany (19162790) is the property of NRC Research Press and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts) 
PEROXIDASE; CADMIUM; OXIDATIVE stress; OXIDATION-reduction reaction; PHYTOREMEDIATION; TRANSGENIC plants; ZINC; PLANT genetic engineering; BOTANY -- Research; ascorbate peroxidase; transgenic Arabidopsis plants; peroxydase ascorbique; phytoremédiation; plants transgénique de l'Arabidopsis; stress oxydatif