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624736 
Journal Article 
Formation and microbial community analysis of chloroanilines-degrading aerobic granules in the sequencing airlift bioreactor 
Zhu, L; Xu, X; Luo, W; Cao, D; Yang, Y 
2008 
Yes 
Journal of Applied Microbiology
ISSN: 1364-5072
EISSN: 1365-2672 
104 
1 
152-160 
WOS:000251670600015 
Aims:  This paper investigates a selection-based acclimation strategy for improving the performance and stability of aerobic granules at a high chloroanilines loading. Methods and Results:  The experiments were conducted in a sequencing airlift bioreactor (SABR) to develop aerobic granules fed with chloroanilines (ClA). The evolution of aerobic granulation was monitored using image analysis and scanning electron microscopy, and PCR–DGGE analysis of microbial community was performed. The sludge granulation was apparently developed by decreased settling time and gradual increased ClA loading to 0·8 kg m−3 day−1. A steady-state performance of the granular SABR was reached at last, as evidenced by biomass concentration of 6·3 g l−1 and constant ClA removal efficiency of 99·9%. The mature granules had a mean size of 1·55 mm, minimal settling velocity of 68·4 m h−1, specific ClA degradation rate of 0·181 g gVSS−1 day−1. Phylogenetic analysis of aerobic ClA-degrading granules confirmed the dominance of β -, γ -Proteobacteria and Flavobacteria. Conclusions:  The chosen operating strategy involving step increase in ClA loading and enhancement of major selection pressures was successful in cultivating the aerobic ClA-degrading granules. Significance and Impact of the Study:  This research could be helpful for improving the stability of aerobic granules via optimizing operating conditions and developing economic feasible full-scale granular bioreactor. [ABSTRACT FROM AUTHOR] Copyright of Journal of Applied Microbiology is the property of Blackwell Publishing Limited and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts) 
CHLOROANILINE; ANILINE; CHLOROBENZENE; MICROBIAL aggregation; MICROBIAL biotechnology; MICROBIAL carcinogenesis; MICROBIAL contamination; PARTICLES (Nuclear physics); IMAGING systems; aerobic granule; chloroanilines; microbial community; operating strategy; sequencing airlift bioreactor