Jump to main content
US EPA
United States Environmental Protection Agency
Search
Search
Main menu
Environmental Topics
Laws & Regulations
About EPA
Health & Environmental Research Online (HERO)
Contact Us
Print
Feedback
Export to File
Search:
This record has one attached file:
Add More Files
Attach File(s):
Display Name for File*:
Save
Citation
Tags
HERO ID
625777
Reference Type
Journal Article
Title
Comparative metabolism and mutagenicity of azo and hydrazone dyes in the Ames test
Author(s)
De France, BF; Carter, MH; Josephy, PD
Year
1986
Is Peer Reviewed?
Yes
Journal
Food and Chemical Toxicology
ISSN:
0278-6915
EISSN:
1873-6351
Volume
24
Issue
2
Page Numbers
165-169
Language
English
PMID
3957165
DOI
10.1016/0278-6915(86)90352-2
Web of Science Id
WOS:A1986A746300014
Abstract
Enteric bacterial and hepatic azoreductase enzymes are capable of reducing azo dyes to yield the constituent aromatic amines. Azo dyes based on benzidine and benzidine congeners have received particular attention because of their widespread use and the known carcinogenicity of benzidine to humans. Azo dyes based on beta-diketone coupling components exist preferentially as the tautomeric hydrazones. A series of hydrazone dyes based on benzidine and benzidine congeners was prepared and characterized by NMR and UV-visible spectroscopy. These dyes were tested for mutagenicity using a modified Ames assay and, unlike the true azo dyes, showed no significant mutagenic activity. The hydrazone dyes were resistant to enzymatic reduction by FMN-supplemented hamster-liver post-mitochondrial supernatant (S-9); under identical conditions, azo dyes such as trypan blue were rapidly reduced.
Tags
OPPT
•
Malonates
Literature Search
Human Health
Toxline (private)
Environmental Hazard
Toxline (private)
Full-text Review
Human Health
Data Evaluation
Human Health
Home
Learn about HERO
Using HERO
Search HERO
Projects in HERO
Risk Assessment
Transparency & Integrity