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6268251 
Journal Article 
Review 
Making Formalin-Fixed, Paraffin Embedded Blocks 
Sadeghipour, A; Babaheidarian, P 
2019 
1 
Methods in Molecular Biology
ISSN: 1064-3745
EISSN: 1940-6029 
1897 
253-268 
English 
30539450 
Paraffin embedding is a standard technique used in clinical and research laboratories to create a formalin-fixed, paraffin-embedded (FFPE) block of tissue. Formalin-fixed tissue undergoes tissue processing and then is embedded in paraffin (wax) to create a FFPE block or paraffin block. The paraffin block can be cut using a microtome to generate thin sections of tissue contained in paraffin to be stained or paraffin tissue ribbons suitable for nucleic acid extraction. In addition, the FFPE blocks can be stored at room temperature for years. Herein, we provide a basic knowledge, and introduce common methods of the paraffin embedding process.