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HERO ID
6282315
Reference Type
Book/Book Chapter
Title
Chapter 3 - Tissue Processing and Staining for Histological Analyses
Author(s)
Qasem, SA; Yousif, MQ
Year
2016
Publisher
Academic Press
Location
Boston
Book Title
Skin Tissue Engineering and Regenerative Medicine
Page Numbers
49-59
DOI
10.1016/B978-0-12-801654-1.00003-6
URL
http://www.sciencedirect.com/science/article/pii/B9780128016541000036
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Abstract
The process of preparing tissue for histological analysis begins with tissue fixation, typically in formalin, followed by processing into paraffin wax, embedding, and cutting thin sections that are affixed to glass slides. Hematoxylin and eosin is the standard stain used for tissue analysis. In addition, there is a long list of histochemical, immunofluorescent, and immunohistochemical stains that aid in differentiating tissue components and identifying certain pathological processes.
Keywords
Embedding; Fixation; Histology; Microscopy; Tissue processing
Editor(s)
Holmes Iv, James H.
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