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628660 
Journal Article 
Arsenic trioxide–loaded, microemulsion-enhanced cytotoxicity on MDAH 2774 ovarian carcinoma cell line 
Terek, MC; Karabulut, B; Selvi, N; Akman, L; Karasulu, Y; Ozguney, I; Sanli, AU; Uslu, R; Ozsaran, A 
2006 
Yes 
International Journal of Gynecological Cancer
ISSN: 1048-891X
EISSN: 15251438 
16 
2 
532-537 
English 
16681722 
WOS:000236943200011 
The antiproliferative effect of As2O3-loaded microemulsion (As2O3-M) on human MDAH 2774 ovarian cancer cells was compared with a regular solution of the As2O3. We used MDAH 2774 as model cell lines for ovarian cancer. The (2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]-2H-tetrazolium hydroxide) (XTT) and trypane blue dye exclusion tests were used to evaluate cytotoxicity. Apoptotic effect of solutions was evaluated using cell death detection kit. Standard microemulsion formulation used in this experiment contains 5 × 10−6 M As2O3. It was clearly demonstrated that As2O3-M had a significant cytotoxic effect on MDAH 2774 cell line, and the cytotoxic effect of As2O3-M was significantly higher than that of regular As2O3 solutions. Even approximately 6000 times diluted microemulsion formulation loaded with 5 × 10−6 M As2O3 showed a cytotoxic effect. As a result, this diluted concentration (approximately 8 × 10−10 M) was found to be approximately 6000 times more effective than regular As2O3 solutions (5 × 10−6 M). Moreover, this diluted concentration resulted in 1.5-fold enhancement of apoptosis. According to the in vitro cytotoxicity studies, we concluded that by incorporating As2O3 into the microemulsion (As2O3-M), which is a new drug carrier system, it is possible to increase antiproliferative effect of regular As2O3 on MDAH 2774 cells. Translating these results to in vivo conditions would open new windows in the treatment of ovarian cancer. 
As2O3-loaded microemulsion; cytotoxicity; ovarian cancer